Page 1 of 8
BI10.1-7 | Introduction to Molecular Biology
Learning Objectives
- Describe the structure and clinical significance of nucleotides and nucleic acids (BI10.1)
- Explain de novo and salvage pathways of purine synthesis, with emphasis on the salvage pathway and its clinical relevance (BI10.2)
- Describe purine degradation, uric acid formation, and associated disorders including gout, Lesch-Nyhan syndrome, and ADA deficiency (BI10.3)
- Outline the major steps of DNA replication, transcription, and translation (BI10.4)
- Describe types of DNA repair mechanisms and their associated disorders, and classify gene mutations (BI10.5)
- Explain the basic mechanisms of gene expression regulation at transcriptional, epigenetic, and post-transcriptional levels (BI10.6)
- Describe applications of recombinant DNA technology, PCR, microarray, FISH, and CRISPR in diagnosis and treatment of diseases (BI10.7)
INSTRUCTIONS
This self-directed learning guide covers Molecular Biology (BI10.1-BI10.7). Read each section carefully, attempt the self-check quizzes before revealing answers, and complete the reflection activity at the end. Estimated reading time: 25-30 minutes.
References
- Harper's Illustrated Biochemistry, 31st Edition — Chapters 34-40 (textbook)
- Lehninger Principles of Biochemistry, 8th Edition — Part III (textbook)
- Vasudevan's Textbook of Biochemistry for Medical Students, 10th Edition (textbook)
- Servier Medical Art — Nucleic acids illustrations (illustration)
Version 2.0 | Faculty review pending